Molecular cell biology / Harvey Lodish . . . [et al.]

Contents Include:

Part I Chemical and molecular foundations

1. Evolution: molecules, genes, cells, and organisms

1.1 The molecules of life
1.2 Prokaryotic cell structure and function
1.3 Eukaryotic cell structure and function
1.4 Unicellar Eukaryotic organisms widely used in cell biology research
1.5 Metazoan structure, function, evolution, and differentiation
1.6 Metazoan organisms widely used in cell biology research \

2. Chemical foundations

2.1 Covalent bonds and noncovalent interactions
2.2 Chemical building blocks of cells
2.3 Chemical reactions and chemical equilibrium
2.4 Biochemical energetics

3. Protein structure and function

3.1 Hierarchical structure of proteins
3.2 Protein folding
3.3 Protein binding and enzyme catalysis
3.4 Regulating protein function
3.5 Purifying, detecting, and characterzing proteins
3.6 Proteomics

4. Culturing and visualizing cells

4.1 Growing and studying cells in culture
4.2 Light microscopy: exploring structure and visualizing proteins within cells
4.3 Electron microscopy: high-resolution imaging
4.4 Isolation of cell organelles


Part II Biomembranes, genes, and gene regulation

5. Fundamental molecular genetic mechanisms

5.1 The double-helical structure of DNA
5.2 DNA replication
5.3 DNA repair and recombination
5.4 Transcription of protein-coding genes and formation of mRNA
5.5 The decoding of mRNA by tRNAs
5.6 Stepwise synthesis of proteins on ribonsomes
5.7 Viruses: parasites of the cellular genetic system

6. Molecular genetic techniques

6.1 Using genetic analysis of mutations to identify and study genes
6.2 DNA cloning and characterization
6.3 Using sequence information to identify genes and deduce their function
6.4 Locating and identifying genes that specify human traits
6.5 Using cloned DNA fragments to study gene expression
6.6 Altering the function of specific genes by design

7. Genes, chromatin, and chromosomes

7.1 Eukaryotic gene structure and organization
7.2 Chromosomal organization of genes and noncoding DNA
7.3 Transposable (mobile) DNA elements
7.4 Structural organization of eukaryotic chromatin and chromosomes
7.5 Morphology and functional elements of eukaryotic chromosomes

8. Transcriptional control of gene expression

8.1 Overview of eukaryotic transcription
8.2 RNA ploymerase II promoters and general transcription factors
8.3 regulatory sequences for protein coding genes and the proteins through which they function
8.4 Molecular mechanisms of transciption repression and activation
8.5 regulation of transciption-factor activity
8.6 Epigenetic regulation of transcription
8.7 Other eukaryotic transcription systems

9. Post-transcriptional gene control

9.1 processing of eukaryotic pre-mRNA
9.2 Regulation of pre-mRNA processing
9.3 Transport of mRNA across the nuclear envelope
9.4 Cyoplasmic mechanisms of post-transcriptional control
9.5 Processing of rRNA and tRNA
9.6 Nuclear bodies are functionally specialized nuclear domains

10. Biomebrane structure

10.1 The lipid bilayer: composition and structural organization
10.2 Membrane proteins: structure and basic functions
10.3 Phospholipids, spingolipids, and cholestrol: synthesis and intracellular movement


Part III Cellular organization and function

11. Transmembrane transport of ions and small molecules

11.1 Overview of transmembrane transport
11.2 Facilitated transport of glucose and water
11.3 ATP-powered pumps and the intracellular ionic environment
11.4 Nongated ion channels and the resting membrane potential
11.5 cotransport by symporters and antiporters
11.6 transcellular transport

12 Cellular energetics

12.1 Chemiosmosis, electron transport, the proton-motive force, and ATP synthesis
12.2 First step of harvesting energy from glucose: glycolysis
12.3 The structure of mitochondria
12.4 The dynamics of mitochondria and mitochondrial- ER membrane contact sites
12.5 The citric acid cycle and fatty acid oxidation
12.6 The electron-transport chain and generation of proton-motive force
12.7 Harnessing the proton-motive force to synthesize ATP
12.8 Chloroplasts and photosynthesis
12.9 Use of light energy to generate molecular oxygen, NADPH, and ATP in stages 1-3 of photosynthesis
12.10 ATP and NADPH drive carbon fixation in the calvin cycle and carbohydrate synthesis in stage 4 of photosynthesis

13 Moving proteins into membranes and organelles

13.1 Targeting proteins to and across the ER membrane
13.2 Insertion of membrane proteins into ER
13.3 Protein modifications, folding, and quality control in the ER
13.4 Targeting of proteins to mitochondria and chloroplasts
13.5 Targeting of peroxisomal proteins
13.6 transport into and out of the nucleus

14 Vesicular traffic, secretion, and endcytosis

14.1 Techniques for studying the secretory pathway
14.2 Molecular mechanisms of vesicle budding and fusion
14.3 Early stages of the secretory pathway
14.4 Later stages of the secretory pathway
14.5 Receptor-mediated endocytosis
14.6 Directing membrane proteins and cytosolic materials to the lysosome for degration

15 Receptors, hormones, and cell signaling

15.1 Signal transduction pathways: from extracelllular signal to cellular response
15.2 Studying cell-surface receptors and signal transduction proteins
15.3 Structure and mechanism of G protein - coupled receptors
15.4 regulating metabolism of many cells: G protein - coupled receptors that activate or inhibit adenylyl cyclase
15.5 Regulating protein secretion and muscle contraction: Ca2+ ions as second messengers in multiple signal transduciton pathways
15.6 Vision: how the eye senses light

16 Growth factor and cytokine signaling pathways that control gene expression

16.1 Growth factors and their receptor tyrosine kinases
16.2 The Ras / MAP kinase signal transduction pathway
16.3 Phosphoinositide signal transduction pathways
16.4 Cytokines, cytokine receptors, and the JAK/ STAT signaling pathway
16.5 The TGF-β family of growth factors, their receptor serine kinases, and the smad transcription factors they activate
16.6 Signal transduction pathways that utilize regulated, sitespecific protein cleavage: notch/ delta and EGF precursors
16.7 Signal transduction pathways that utilize proteasomal degradation of signaling components: Wnt, hedgehog, and the many hormones that activate NF-κB

17 Cell organization and movement I: microfilaments

17.1 Microfilaments and actin structures
17.2 Dynamics of actin filaments
17.3 Mechanisms of actin filaments assembly
17.4 Organization of actin-based cellular structures
17.5 Myosins actin-based motor proteins
17.6 Myosin-powered movements
17.7 Cell migration: mechanisms, signaling, and chemotaxis

18 Cell organization and movement II: microtubules and intermediate filaments

18.1 Microtubule structure and organization
18.2 Microtubule dynamics
18.3 Regulation of microtubule structure and dynamics
18.4 Kinesins and dyneins: microtubule-based motor proteins
18.5 Cilia and flagella: microtubule-based surface structures
18.6 Mitosis
18.7 Intermediate filaments
18.8 Coordination and cooperation between cytoskeletal elements

19 The Eukaryotic cell cycle

19.1 Overview of the cell cycle
19.2 Model organisms and methods of studying the cellcycle
19.3 Cell cycle progression and control: feedback loops and post-translational modification
19.4 The transition from G1 into S phase and DNA replication
19.5 The G2/M transition and the irreversible engine of mitosis
19.6 The mitotic spindle, chromosome segregation, and exit from mitosis
19.7 Surveillance mechanisms in cell cycle regulation
19.8 Meiosis: a special type of cell division

Part IV Cell growth and differenciation

20 Integrating cells into tissues

20.1 Cell-cell and cell- extracellular matrix adhesion: an overview
20.2 Cell-cell and cell - extracellular matrix junctions and their adhesion molecules
20.3 The extracellular matrix I: the basal lamina
20.4 The extracellular matrix II: connective tissue
20.5 Adhesive interactions in motile and nonmotile cells
20.6 plant tissues

21 Responding to the cellular environment

21.1 Regulating blood glucose level
21.2 Integrating cell growth signals with nutrient and energy levels
21.3 Responding to changes in the levels of cholesterol and unsaturated fatty acids
21.4 Responding to low oxygen
21.5 Responding to elevated temperatures
21.6 Sensing night and day: circadian rhythms
21.7 Sensing and responding to the physical environment

22 Stem cells, cell asymmetry, and regulated cell death

22.1 Early mammalian development, embryonic stem cells and induced pluripotent stem cells
22.2 Stem cells and niches in multicellular organisms
22.3 Mechanisms of cell polarity and asymmetric cell division
22.4 Cell death and its regulation

23 Cells of the nervous system

23.1 Neurons and glia: building blocks of the nervous system
23.2 Voltage-gated ion channels and the propagation of action potentials
23.3 Communication at synapses
23.4 Sensing the environment: touch, pain, taste, and smell
23.5 Forming and storing memories

24 Immunology

24.1 Overview of host defenses
24.2 Immunoglobulins: structure and function
24.3 Generation of antibody diversity and B-cell development
24.4 The MHC and antigen presentation
24.5 T Cells, T-cell receptors, and T-cell development
24.6 Collaboration of immune-system cells in the adaptive response

25 Cancer

25.1 How tumor cells differ from normal cells
25.2 Genetic and genomic basis of cancer
25.3 Dysregulation of cell growth and developmental pathways initiates tumorigenesis
25.4 Evasion of programmed cell death and immune surveillance processes

Includes bibliographical references and index.